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Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low Serum
Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low Serum
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貨期:
編號:B192288
品牌:Mingzhoubio

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  • 產品名稱 Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low Serum
    商品貨號 B192288
    Biosafety Level 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Product Format frozen 1 Kit
    Storage Conditions -20°C (or -70°C for long-term storage)
    Comments
    Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low Serum contains components that when added to Mesenchymal Stem Cell Basal Medium (ATCC PCS-500-030) create a complete cell culture environment for mesenchymal stem cells isolated from normal human adipose and umbilical tissue. The final concentration for each component in complete mesenchymal stem cell growth medium is as follows:
    • rh FGF basic: 5 ng/mL
    • rh FGF acidic: 5 ng/mL
    • rh EGF: 5 ng/mL
    • Fetal Bovine Serum: 2%
    • L-Alanyl-L-Glutamine: 2.4 mM
    Complete Growth Medium
    1. Obtain one Mesenchymal Stem Cell Growth Kit– for Adipose and Umbilical-derived MSCs - Low serum from the freezer; make sure that the caps of all components are tight.
    2. Thaw the components of the growth kit just prior to adding them to the basal medium.
    3. Obtain one bottle of Mesenchymal Stem Cell Basal Medium (485 mL) from cold storage.
    4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
    5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer.
    6. Table 1. Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low Serum Components

      Component                     

      Volume

      Volume

      Sterility Tests

      Final Concentration

      Functional Tests

      MSC Supplement

      C of A

      10 mL

      梅經理 17280875617 1438578920
      胡經理 13345964880 2438244627
      周經理 17757487661 1296385441
      于經理 18067160830 2088210172
      沈經理 19548299266 2662369050
      李經理 13626845108 972239479
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