Restriction digests of the clone give the following sizes (kb): EcoRI--6.6; SmaI--6.6; BamHI--6.6; SalI--6.6; PstI--6.6. bleR = bleomycin-pheomycin resistance One of a series (ATCC 37637 - 37639) of 3 plasmid vectors for characterization of regulatory systems in Bacillus subtilis. Inserted genes can be fused to Escherichia coli lacZ, with one vector in the series for each of the 3 translational reading frames. A 3.1 kb EcoRI/DraI fragment of pNM481 was ligated to a 3.5 kb EcoRI/PvuII fragment of pUB110. |
