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pHRL83-IVS4
pHRL83-IVS4
規格:
價格:詢價
編號:B192501
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pHRL83-IVS4
商品貨號
B192501
Designations
pHRL83-IVS4
Species
Homo sapiens, human
培養基
Depositors
LH Kedes
Vector
Construct size (kb): 4.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 0.4000000059604645
Gene product: actin, alpha, cardiac muscle [ACTC]
Insert Size (kb)
0.400
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; HindIII--4.8; BamHI--4.8; PstI--4.4, 0.4.
This probe hybridizes to human but not rodent cardiac actin sequences under the following conditions: 4X SSC; 50 mM NaH2P04; 5 X Denhardts, 10% dextran sulfate at 65C. Wash at 0.5X SSC, 0.1% SDS, 65 C.
The insert corresponds to intervening sequence 4 of the gene.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
References

Gunning P, et al. Expression of human cardiac actin in mouse L cells: A sarcomeric actin associates with a nonmuscle cytoskeleton. Cell 36: 709-715, 1984. PubMed: 6538118

Gunning P, et al. Chromosomal location of the co-expressed human skeletal and cardiac actin genes. Proc. Natl. Acad. Sci. USA 81: 1813-1817, 1984. PubMed: 6584914

Barbara Weiffenbach, personal communication

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