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lambdaADH
lambdaADH
規格:
貨期:
編號:B192504
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
lambdaADH
商品貨號
B192504
Designations
lambdaADH
Depositors
SJ Elledge
Biosafety Level
1
Vector Information
Size (kb): 42.40
DESCRIPTION OF VECTOR:
Intact vector size: 42.400
Type of vector: phage
Cloning sites: XhoI EcoRI
Polylinker sites: BglII EcoRI BamHI XhoI BglII
Cloning capacity: 0 - 8.4 kb
Construction: lambdaYES-R, pADH
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: ADH, ->
MCS: XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): URA3, ->
centromere: CEN4
other: right arm (15.2 kb, with cI857)
Vector: lambdaADH (phage, lambda - replacement)
Promoters: Promoter for expression ADH
Construction: lambdaYES-R, pADH
Marker(s):URA3,ampR
Construct size (kb): 42.40
Features: marker(s): URA3
marker(s): ampR
other: left arm (19.6 kb)
other: lox
other: right arm (15.2 kb, with cI857)
promoter for expression: ADH
promoter for expression: lac
replicon: ARS1
replicon: pMB1
MCS: XhoI EcoRI
transcription terminator: HIS3
centromere: CEN4
Applications
expression vector
lambda vector allowing conversion to plasmid
vector for constructing cDNA libraries
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--43.0; SalI--21.0, 13.5, 13.0; XhoI--25.0, 21.0.
Transcription initiated from the ADH promoter in yeast hosts can be terminated at the HIS3 terminators.
Vector for the construction of cDNA libraries permitting regulated expression in both yeast and E. coli and simple conversion to plasmid via the cre-lox system.
The plasmid derivative (pADH, 7.75 kb) is a YC-type shuttle expression vector.
The lac promoter contains a ribosome binding sequence optimally spaced from an AUG start codon and can support translational fusions.
Growth Conditions
Temperature: 37.0°C
References

Stephen J Elledge, personal communication

Shipped
frozen
Shipping Information
Distributed: freeze-dried bacteria-free lysate
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