| 產品名稱 |
pHUGP13 [pGEM4-HUGP-13] |
| 商品貨號 |
B192708 |
| Designations |
pHUGP13 [pGEM4-HUGP-13] |
| GenBank Number |
M15182
|
| Species |
Homo sapiens, human |
| Depositors |
WS Sly |
| Vector |
Construct size (kb): 5.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-4
Intact vector size: 2.870
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: EcoRI...HindIII, ->
promoter for in vitro transcription: T7, <-
replicon: pMB1
marker(s): ampR, <-
promoter for in vitro transcription: SP6, ->
Cross references: DNA Seq. Acc.: X65303 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GUSB
Genomic copy number: unique
Gene name: glucuronidase, beta
Contains complete coding sequence?: Y
Chromosome: 7; Localization: 7 q22
Tissue: placenta
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 2.191
Cross references: DNA Seq. Acc.: M15182
Nucleotides 1-2191 of the insert correspond to
nucleotides 1-2191 of M15182. Insert lengths(kb): 2.190999984741211 Tissue: placenta Gene product: glucuronidase, beta(beta-glucuronidase) [GUSB] |
| Insert Size (kb) |
2.191 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.5, 1.1, 0.72; BamHI/HindIII--3.5, 1.1, 0.72; EcoRI--2.9, 2.3; HindIII--5.2; PstI--3.6, 0.88; 0.62. The insert contains the following restriction sites (approximate kb from the 5' end): NotI--0.2; PstI--0.1, 0.67, 1.58; BamHI--1.0, 1.68. |
| References |
Oshima A, et al. Cloning, sequencing, and expression of cDNA for human beta-glucuronidase. Proc. Natl. Acad. Sci. USA 84: 685-689, 1987. PubMed: 3468507
|
