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pCMV5Y17
pCMV5Y17
規格:
價格:
編號:B192744
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCMV5Y17
商品貨號
B192744
Designations
pCMV5Y17
GenBank Number

J02933

培養基
Species
Homo sapiens, human
Depositors
W Cheung
Applications
in another host, produces protein coagulation factor VII (serum prothrombin conversion accelerator)
Vector
Construct size (kb): 6.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCMV5
Intact vector size: 4.700
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI BamHI HindIII
Polylinker sites:
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
promoter: CMV, ->
terminator: hGH, ->
replicon: SV40
replicon: pMB1
marker(s): ampR, <-
replicon: f1, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: F7
Genomic copy number: unique
Gene name: coagulation factor VII (serum prothrombin conversion
accelerator)
Contains complete coding sequence?: U
Chromosome: 13; Localization: 13 q34
Type of DNA: cDNA
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 1.4
Cross references: DNA Seq. Acc.: J02933
Insert lengths(kb): 1.399999976158142
Gene product: coagulation factor VII (serum prothrombin conversion accelerator) [F7]
Target Gene: coagulation factor VII (serum prothrombin conversion accelerator)
Insert Size (kb)
1.400
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.7, 1.4; EcoRI/BstEII--5.8, 0.3; PstI--4.7, 0.65, 0.35 (doublet); SacI--5.0, 0.7, 0.4; PvuII--2.5, 1.4, 1.3, 0.6, 0.25.
Digestion with EcoRI+BstEII produces fragments of 5.75 and 0.35 kb.
Bacteria may grow slowly due to the high plasmid copy number.
Encodes the following mutant form: A1Y, K32E, D33N, A34T, K38T, L39E.
References

Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778

Cheung WF, et al. The binding of human factor IX to endothelial cells is mediated by residues 3-11. J. Biol. Chem. 267: 20529-20531, 1992. PubMed: 1400370

Toomey JR, et al. Localization of the human tissue factor recognition determinant of human factor VIIa. J. Biol. Chem. 266: 19198-19202, 1991. PubMed: 1918037

Wing-Fai Cheung, personal communication

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