| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--13.0, 7.4, 6.5, 5.0, 4.2, 3.7, 0.7; HindIII--7.4, 7.4, 6.5, 5.9, 3.6, 2.6, 2.1, 1.95, 0.7; BglII--11.0, 6.5, 5.8, 4.3, 4.1, 2.7, 0.7; PstI--6.5, 5.0, 3.6, 2.8, 2.3, 2.1, 2.0, 1.6, 1.5, 1.4, 1.2, 1.1, 1.0, 0.8, 0.6, 0.5. Insert fragments can be separated from the vector with an EcoRI digest. The vector fragment is 5.5 kb. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: PstI (panel of 5 individuals). |
| References |
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
Bowden DW, et al. Identification and characterization of 23 RFLP loci by screening random cosmid genomic clones. Am. J. Hum. Genet. 44: 671-678, 1989. PubMed: 2565079
Weiffenbach B, et al. A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10: 173-185, 1991. PubMed: 1675192
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