contains easily purifiable cassette(s) for construction
Comments
Restriction digests analyzed on agarose gels give the following sizes (kb): EcoRI--2.2; EcoRI/PstI-- 1.5, 0.7.
Source of Escherichia coli lacZ promoter and ribosome binding site for use in constructing expression vectors.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Lauer G, et al. Construction of overproducers of the bacteriophage 434 repressor and cro proteins. J. Mol. Appl. Genet. 1: 139-147, 1981. PubMed: 6286819
