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pAL3b [P3, pAL3]

規(guī)格:

價(jià)格:

編號(hào):B194549

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pAL3b [P3, pAL3]

商品貨號(hào)

B194549

Designations

pAL3b [P3, pAL3]

GenBank Number

M14218

培養(yǎng)基

Species

Homo sapiens, human

Depositors

WE O'Brien

Vector

Construct size (kb): 4.300000190734863

Insert

DNA: cDNA

Insert lengths(kb): 1.565000057220459

Tissue: liver

Gene product: argininosuccinate lyase [ASL]

Insert Size (kb)

1.565

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 0.96, 0.7; HindIII--2.8, 1.5; PstI--3.7 + others; SmaI--3.5, 0.6; BamHI--2.7, 1.65.

The 5' EcoRI fragment also hybridizes to a 4 kb EcoRI fragment from chromosome 22. The 3' EcoRI fragment hybridizes to chromosome 7 only.

Includes the complete coding region for a protein of 463 aa with 115 bp of 5' untranslated sequence, 46 bp of 3' untranslated sequence, and a polyadenylation signal of AATAAA 16 bp from a poly(A) tail of 17 bp.

References

O'Brien WE, et al. Cloning and sequence analysis of cDNA for human argininosuccinate lyase. Proc. Natl. Acad. Sci. USA 83: 7211-7215, 1986. PubMed: 3463959

B214693：pAL3b [P3, pAL3]

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