| 產品名稱 |
pAIIe/6 |
| 商品貨號 |
B194610 |
| Designations |
pAIIe/6 |
| GenBank Number |
J04695
|
| Species |
Mus musculus, mouse |
| Depositors |
I Oberbaumer |
| Applications |
The internal 0.8 StuI fragment is recommended for hybridization. |
| Vector |
Construct size (kb): 3.599999904632568 |
| Insert |
DNA: cDNA Insert lengths(kb): 0.8799999952316284 Tissue: teratocarcinoma PYS-2/12 cell line Gene product: collagen, type IV, alpha 2 [Col4a2] |
| Insert Size (kb) |
0.880 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: frozen |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.72, 0.88; EcoRI--3.6; BamHI--3.5, 0.1; PstI--3.6; SalI--3.6. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.01; KpnI--0.02; StuI--0.03, 0.87; PvuII--0.07; SmaI--0.23; BglI--0.34, 0.66. Extends from nt 2331 to 3216 of the reported sequence, encoding part of the triple helical domain. The internal 0.8 StuI fragment is recommended for hybridization. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975
Saus J, et al. The complete primary structure of mouse alpha2(IV) collagen. J. Biol. Chem. 264: 6318-6324, 1989. PubMed: 2703491
Brazel D, et al. Human basement membrane collagen (type IV)-- The amino acid sequence of the alpha2(IV) chain and its comparison with the alpha1(IV) chain reveals deletions in the alpha1(IV) chain. Eur. J. Biochem. 172: 35-42, 1988. PubMed: 3345760
Ilse Oberbaumer, personal communication
|
