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pAIIe/6
pAIIe/6
規(guī)格:
價格:
編號:B194610
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pAIIe/6
商品貨號
B194610
Designations
pAIIe/6
GenBank Number

J04695

培養(yǎng)基
Species
Mus musculus, mouse
Depositors
I Oberbaumer
Applications
The internal 0.8 StuI fragment is recommended for hybridization.
Vector
Construct size (kb): 3.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 0.8799999952316284
Tissue: teratocarcinoma PYS-2/12 cell line
Gene product: collagen, type IV, alpha 2 [Col4a2]
Insert Size (kb)
0.880
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: frozen
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.72, 0.88; EcoRI--3.6; BamHI--3.5, 0.1; PstI--3.6; SalI--3.6.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.01; KpnI--0.02; StuI--0.03, 0.87; PvuII--0.07; SmaI--0.23; BglI--0.34, 0.66.
Extends from nt 2331 to 3216 of the reported sequence, encoding part of the triple helical domain.
The internal 0.8 StuI fragment is recommended for hybridization.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975

Saus J, et al. The complete primary structure of mouse alpha2(IV) collagen. J. Biol. Chem. 264: 6318-6324, 1989. PubMed: 2703491

Brazel D, et al. Human basement membrane collagen (type IV)-- The amino acid sequence of the alpha2(IV) chain and its comparison with the alpha1(IV) chain reveals deletions in the alpha1(IV) chain. Eur. J. Biochem. 172: 35-42, 1988. PubMed: 3345760

Ilse Oberbaumer, personal communication

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