| 產品名稱 |
pG36/T1 [B237/L2-T1] |
| 商品貨號 |
B195206 |
| Designations |
pG36/T1 [B237/L2-T1] |
| Species |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors |
A Tzagoloff |
| Applications |
The insert includes a 1.89 kb open reading frame encoding a 74 kDa basic protein essential for an early step in splicing the cytochrome b pre-mRNA. |
| Vector |
Construct size (kb): 21.70000076293945 |
| Insert |
DNA: genomic Insert lengths(kb): 11.0 Gene product: intron processing factor [CBP2] |
| Insert Size (kb) |
11.0 |
| Genotype |
MATalpha leu2-3 leu2-112 cbp2-1 |
| Mating Type |
alpha |
| Media |
ATCC® Medium 1688: Glycerol enriched medium
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
The insert contains the following sites separated by (kb) (approximate): BamHI- 2.4- BamHI- 0.33- EcoRI- 0.55- HindIII. Carries plasmid pG36/T1 with wild-type gene The probe, pG36/T1, has been deposited in Escherichia coli RR1 as ATCC 37662 and in Saccharomyces cerevisiae B237/L2 as ATCC 66318. |
| Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References |
McGraw P, Tzagoloff A. Assembly of the mitochondrial membrane system. Characterization of a yeast nuclear gene involved in the processing of the cytochrome b pre-mRNA. J. Biol. Chem. 258: 9459-9468, 1983. PubMed: 6348045
|