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Rabies virus
Rabies virus
規格:
貨期:
編號:B195420
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Rabies virus
商品貨號
B195420
Classification
Rhabdoviridae, Lyssavirus
Agent
Rabies virus
Strain
CVS-27
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
Deposited as a mouse brain preparation from Seligmann. Tissue culture adapted by Torres-Anjel in following lot. Challenge strain according to Division of Biological Standards and Quality Control.
Effect on Host
No CPE produced on production host.
Recommended Host
Neuro-2a (ATCC® CCL-131™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 50% confluent.   Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1 hour at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.
Incubation: 4 days
Name of Depositor
EB Seligmann Jr., National Institutes of Health, MJ Torres-Anjel, University of Missouri
Source
Derived from Pasteur strain
References

Fernandes MV, et al. Mechanism of the cytopahic effect of Rabies virus in tissue culture. Virology 21: 128-131, 1963. PubMed: 14062905

Kissling RE. Growth of rabies virus in non-nervous tissue culture. Proc. Soc. Exp. Biol. Med. 98: 223-225, 1958. PubMed: 13554599

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