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D-157
D-157
規格:
價格:
編號:B195733
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
D-157
商品貨號
B195733
Designations
D-157
GenBank Number

M85168

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 4.760000228881836
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-3Zf(+)
Intact vector size: 3.199
Type of vector: phagemid
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: T7-EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII
PstI SphI HindIII-SP6
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: AGL
Genomic copy number: unique
Gene name: amylo-1,6-glucosidase, 4-alpha-glucanotransferase (glycogen
debranching enzyme, glycogen storage disease type III)
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 p21
Tissue: muscle
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.563
Cross references: DNA Seq. Acc.: M85168
Insert lengths(kb): 1.562999963760376
Tissue: muscle
Gene product: amylo-1,6-glucosidase, 4-alpha-glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III) [AGL]
Insert Size (kb)
1.563
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.2, 1.0, 0.5; HindIII--3.2, 1.1, 0.4; SacI/PstI--4.7; BamHI--4.0, 0.6; BglII--4.7; XbaI--4.55, 0.25.
The insert contains the following restriction sites (kb from the 5' end): BamHI--0.84; BglII--0.72; EcoRI--1.42; HindIII--0.39; NcoI--0.27, 0.36; XbaI--1.28.
Corresponds to nt 954-2490 of the published sequence, containing part of the coding region.
References

Yang BZ, et al. Molecular cloning and nucleotide sequence of cDNA encoding human muscle glycogen debranching enzyme. J. Biol. Chem. 267: 9294-9299, 1992. PubMed: 1374391

Yuan-Tsong Chen, personal communication

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