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pLUCS Phagemid in Escherichia coli HB101

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編號:B195743

品牌:Mingzhoubio

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產品名稱

pLUCS Phagemid in Escherichia coli HB101

商品貨號

B195743

Designations

pLUCS Phagemid in Escherichia coli HB101

Depositors

A Ward

培養基

Biosafety Level

Vector Information

Size (kb): 5.40

Vector: pLUCS (phagemid)

Promoters: Promoter lac

Construction: pSVOALdelta5', pBluescriptSK+

Marker(s):ampR

Construct size (kb): 5.40

Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1, f1
terminator: SV40 polyadenylation

Applications

promoter-cloning vector

vector containing primer sites useful for sequencing

vector permitting production of single-stranded DNA

vector permitting visual detection of recombinants

Comments

Restriction digests of the clone give the following sizes (kb): AccI--5.4; SalI--5.4; XhoI--5.4.

The AUG start codon is 22bp downstream from the HindIII site within the luc gene.

Permits beta-galactosidase blue/white color selection for identification of recombinants. The color is weaker than from the parent vector and transformed cells may need to be grown 18-24 hours at 37C.

Promoter-cloning vector for analysis of eukaryotic promoters, using the luciferase reporter gene.

Constructed from pLUC (ATCC 77414) by substitution of a StuI restriction site for the HindIII, PstI and BamHI sites in the polylinker.

The order of the major features in the phagemid is: 3' lacZ - XhoI/MCS/StuI - 5' luc 3' - SV40 small t intron - SV40 polyadenylation - NotI - SstII - 5' lacZ - pMB1 ori - ampR - f1 ori.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

de Wet JR, et al. Firefly luciferease gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737, 1987. PubMed: 3821727

Caricasole A, Ward A. A luciferase-reporter vector with blue-white selection for rapid subcloning and mutational analysis of eukaryotic promoters. Gene 124: 139-140, 1993. PubMed: 8382654

Caricasole A, Ward A. Transactivation of mouse insulin-like growth factor II (TGF-II) gene promoters by the AP-1 complex. Nucleic Acids Res. 21: 1873-1879, 1993. PubMed: 8493103

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freeze-dried

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