| 產(chǎn)品名稱 |
Sp3 |
| 商品貨號 |
B195797 |
| Designations |
Sp3 |
| GenBank Number |
M97191
|
| Species |
Homo sapiens, human |
| Depositors |
A Winoto |
| Applications |
contains sequence Sp3 transcription factor |
| Vector |
Construct size (kb): 4.900000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSP72
Intact vector size: 2.462
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: XhoI PvuII HindIII SphI PstI SalI AccI XbaI BamHI SmaI KpnI
SacI EcoRI ClaI EcoRV BglII
Polylinker sites: XhoI PvuII HindIII SphI PstI SalI AccI XbaI BamHI SmaI KpnI
SacI EcoRI ClaI EcoRV BglII
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SP6, T7
replicon: pMB1
Cross references: DNA Seq. Acc.: X65332 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SP3
Gene name: Sp3 transcription factor
Contains complete coding sequence?: Y
Tissue: Hut78 cell line, PMA activated
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 2.4
Cross references: DNA Seq. Acc.: M97191
Nucleotides 1-2221 of the insert correspond to
nucleotides 1-2221 of M97191. Insert lengths(kb): 2.400000095367432 Tissue: Hut78 cell line, PMA activated Gene product: Sp3 transcription factor [SP3] Target Gene: Sp3 transcription factor |
| Insert Size (kb) |
2.400 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglII--3.8, 0.96; EcoRI--2.5, 2.2; NotI--4.7; PstI--3.0, 0.8, 0.6, 0.3; XbaI--4.7. Insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.89; EcoRI--0.0, 2.22; EcoRV--2.07; KpnI--1.63; NcoI--1.06; NotI--0.04; PstI--0.66, 1.43, 1.98; PvuII--1.12; SacI--1.71; XbaI--0.62. |
| References |
Kingsley C, Winoto A. Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression. Mol. Cell. Biol. 12: 4251-4261, 1992. PubMed: 1341900
|
