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Sp3
Sp3
規格:
價格:
編號:B195797
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Sp3
商品貨號
B195797
Designations
Sp3
GenBank Number

M97191

培養基
Species
Homo sapiens, human
Depositors
A Winoto
Applications
contains sequence Sp3 transcription factor
Vector
Construct size (kb): 4.900000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSP72
Intact vector size: 2.462
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: XhoI PvuII HindIII SphI PstI SalI AccI XbaI BamHI SmaI KpnI
SacI EcoRI ClaI EcoRV BglII
Polylinker sites: XhoI PvuII HindIII SphI PstI SalI AccI XbaI BamHI SmaI KpnI
SacI EcoRI ClaI EcoRV BglII
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SP6, T7
replicon: pMB1
Cross references: DNA Seq. Acc.: X65332
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SP3
Gene name: Sp3 transcription factor
Contains complete coding sequence?: Y
Tissue: Hut78 cell line, PMA activated
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 2.4
Cross references: DNA Seq. Acc.: M97191
Nucleotides 1-2221 of the insert correspond to
nucleotides 1-2221 of M97191.
Insert lengths(kb): 2.400000095367432
Tissue: Hut78 cell line, PMA activated
Gene product: Sp3 transcription factor [SP3]
Target Gene: Sp3 transcription factor
Insert Size (kb)
2.400
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglII--3.8, 0.96; EcoRI--2.5, 2.2; NotI--4.7; PstI--3.0, 0.8, 0.6, 0.3; XbaI--4.7.
Insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.89; EcoRI--0.0, 2.22; EcoRV--2.07; KpnI--1.63; NcoI--1.06; NotI--0.04; PstI--0.66, 1.43, 1.98; PvuII--1.12; SacI--1.71; XbaI--0.62.
References

Kingsley C, Winoto A. Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression. Mol. Cell. Biol. 12: 4251-4261, 1992. PubMed: 1341900

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