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A 5.2 kb BamHI(5')/ClaI(3') fragment containing exons 2 and 3 has been used as a probe.

Constructed by ligating a SmaI/EcoRI fragment from a genomic subclone of the gene to a SmaI/EcoRI fragment of pA9 (containing the glucocorticoid control region, MMTV promoter and cap site).

The EcoRI site used in subcloning was derived from the vector (pBR322).

Construct size (kb): 14.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pA9
Intact vector size:
Type of vector: plasmid
Vector end: SmaI
Vector end: EcoRI
Cloning sites: SmaI EcoRI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: MMTV LTR
marker(s): ampR
promoter: MMTV LTR
Cross references:

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: mouse
Gene symbol: Myc
Genomic copy number: unique
Gene name: avian myelocytomatosis viral (v-myc) oncogene homolog
Contains complete coding sequence?: U
Chromosome: 15; Localization: 15
Type of DNA: genomic
Insert 5' end: SmaI
Insert 3' end: EcoRI
Insert size (kb):
Cross references:

Gene product: avian myelocytomatosis viral (v-myc) oncogene homolog( avian myelocytomatosis viral (v-myc) oncogene homolog, proto-oncogene c-myc) [Myc]

Restriction digests of the clone give the following sizes (kb): PstI--4.5, 4.2, 2.5, 2.0, 0.45; PvuII--5.8, 4.0, 1.75, 1.35, 0.54; EcoRI--14.0; HindIII--7.8, 4.4, 1.15, 0.72; BamHI--9.0, 2.4, 2.0, 1.2; BamHI+HindIII--4.4, 3.8, 2.4, 1.2 (doublet), 0.8;

ClaI--9.0, 5.6.

There is more than one SmaI site within the myc gene.

A 5.2 kb BamHI(5')/ClaI(3') fragment containing exons 2 and 3 has been used as a probe. The BamHI site is in intron 1 and the ClaI site is from the flanking pBR322-derived sequences.

This clone is incorrectly cited in U.S. Patent 4,736,866 as ATCC 39746.

The insert begins approximately 1.0 kb 5' of exon 1. It therefore contains the 2 promoters naturally preceding the unactivated gene.

Constructed by ligating a SmaI/EcoRI fragment from a genomic subclone of the gene to a SmaI/EcoRI fragment of pA9 (containing the glucocorticoid control region, MMTV promoter and cap site).

The 3' end of the myc sequence in this construct is the HindIII site approximately 1.0 kb 3' of the poly(A) addition site. The EcoRI site used in subcloning was derived from the vector (pBR322).