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pCu426CUP1
pCu426CUP1
規(guī)格:
價(jià)格:
編號:B196626
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pCu426CUP1
商品貨號
B196626
Designations
pCu426CUP1
Depositors
S Labbe, DJ Thiele
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 6.213
DESCRIPTION OF VECTOR:
Intact vector size: 6.213
Type of vector: phagemid
Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI
Polylinker sites: SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI Xho
Construction: pRS426, CUP1 promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->
replicon: f1, <-
terminator: CYC1, <-
MCS: SpeI...XhoI, ->
promoter for expression: CUP1, ->
replicon: pMB1
marker(s): ampR, <-
replicon: 2 micron, <-
Vector: pCu426CUP1 (phagemid)
Promoters: Promoter for expression CUP1
Construction: pRS426, CUP1 promoter
Marker(s):URA3,ampR
Construct size (kb): 6.212999820709229
Features: marker(s): URA3
marker(s): ampR
promoter for expression: CUP1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: SpeI...XhoI
terminator: CYC1
Applications
cloning vector
Comments
Restriction digests of the vector give the following sizes (kb): SacI--6.2; SacI/SpeI--6.0, 0.3.
Gene CUP1 is the most potent for copper induction. The CUP1 promoter can be used to creat a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae.
References

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452

Related Products
component of:ATCC 87778
Shipped
dried
Shipping Information
Distributed: DNA supplied by depositor (frozen) (amount: 100 ng)
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