| 產品名稱 |
pBS39 [BSY90] |
| 商品貨號 |
B196631 |
| Designations |
pBS39 [BSY90] |
| GenBank Number |
X03453
|
| Species |
Staphylococcus aureus subsp.aureus bacteriophage P1 |
| Depositors |
DuPont Merck Pharmaceutical Company, BL Sauer, DuPont Merck Pharmaceutical Company |
| Applications |
in another host, produces protein recombinase, site-specific |
| Vector |
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Vector end: SalI
Vector end: XhoI
Cloning sites:
Polylinker sites:
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, URA3
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: bacteriophage P1
Gene symbol: cre
Gene name: recombinase, site-specific
Contains complete coding sequence?: U
Type of DNA: genomic
Insert 5' end: XhoI
Insert 3' end: SalI
Insert size (kb): 1.2
Cross references: DNA Seq. Acc.: X03453 Insert lengths(kb): 1.200000047683716 Gene product: recombinase, site-specific [cre] Target Gene: recombinase, site-specific |
| Insert Size (kb) |
1.200 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): SalI/XhoI--4.1 (doublet), 1.5; SalI--9.6; XhoI--5.5, 4.1. Permits inducible expression of cre in S. cerevisiae regulated by the GAL1 promoter. The order of the major features in this plasmid is: ampR - GAL1 promoter - cre - URA3 - ARS1 - CEN4. |
| References |
Sauer BL. Site-specific recombination of DNA in eukaryotic cells. US Patent 4,959,317 dated Sep 25 1990
Sauer B. Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 7: 2087-2096, 1987. PubMed: 3037344
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
