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pYNM67
pYNM67
規格:
價格:
編號:B196958
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pYNM67
商品貨號
B196958
Designations
pYNM67
Species
Homo sapiens, human
培養基
Depositors
RL White, Y Nakamura
Applications
This probe has some lane background, but RFLPs observed under normal conditions of hybridization and washing.
Vector
Construct size (kb): 6.5
Insert
DNA: genomic
Insert lengths(kb): 1.600000023841858
Gene product: DNA Segment, single copy [D17S29]
Alleles: B1, A1, A1, B1, B2, B2, C1, C1, C2, C2, A2, A2
Insert Size (kb)
1.600
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.5; PstI--4.7, 1.6; HindIII--6.5; BamHI--6.5; ClaI--6.5.
Codominant segregation for the TaqI RFLP demonstrated in 46 3-generation families. Codominant segregation demonstrated in 40 3-generation families for the RsaI RFLP.
RsaI also identifies two additional polymorphisms. TaqI detects a 2-allele RFLP.
This probe has some lane background, but RFLPs observed under normal conditions of hybridization and washing.
Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, MspI, PstI, PvuII.
References

Baker SJ, et al. Chromosome 17 deletions and p53 gene mutations in colorectal carcinomas. Science 244: 217-221, 1989. PubMed: 2649981

Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050

Yusuke Nakamura, personal communication

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