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HHCMD33 [D17S2061, UTR-9804]
HHCMD33 [D17S2061, UTR-9804]
規格:
價格:
編號:B197204
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HHCMD33 [D17S2061, UTR-9804]
商品貨號
B197204
Designations
HHCMD33 [D17S2061, UTR-9804]
GenBank Number

M34175

M78004

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 5.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ADTB*
Gene name: adaptin, beta
Contains complete coding sequence?: N
Chromosome: 17; Localization: 17, cR
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed
(Stratagene 936205)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.3
Cross references: DNA Seq. Acc.: M34175; EST: EST01589
Nucleotides 1-207, 207-319 of the insert correspond to
nucleotides 835-1041, 1040-1152 of M34175 with 99% identity.
DNA Seq. Acc.: M78004; EST: EST01589; Primer: M13 Forward;
THC id: THC125091
Insert lengths(kb): 2.299999952316284
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205)
Gene product: adaptin, beta [ADTB*]
Insert Size (kb)
2.300
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.4, 0.9.
The insert contains multiple EcoRI fragments. It is important to note these may have been derived from different cDNAs.
Based on the size of the insert and the alignment with GenBank accession M34175 (5701 nt, CDS178-2991), this clone probably contains part of the coding sequence and part of the 3' untranslated region.
References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

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