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pMCT128.1 [MCT128.1]
pMCT128.1 [MCT128.1]
規(guī)格:
貨期:
編號(hào):B197707
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pMCT128.1 [MCT128.1]
商品貨號(hào)
B197707
Designations
pMCT128.1 [MCT128.1]
Species
Homo sapiens, human
Depositors
RL White, Y Nakamura
Applications
Otherwise, RFLPs were observed under normal hybridization and wash conditions.
Vector
Construct size (kb): 6.0
Insert
DNA: genomic
Insert lengths(kb): 3.0
Gene product: DNA Segment, single copy [D11S144]
Alleles: A1, A1, A1, A2, A2, A2
Insert Size (kb)
3.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--2.7, 1.2, 0.94, 0.74; PstI--3.6, 1.7, 0.6; EcoRI/HindIII--3.0, 1.7, 0.82, 0.5; EcoRI--4.25, 0.87, 0.43.
This probe was also known as D11S285.
Codominant segregation demonstrated in 46 3-generation families.
This probe was pre-associated with excess human DNA before hybridization. Otherwise, RFLPs were observed under normal hybridization and wash conditions.
Enzyme(s) not detecting polymorphism: BglII, PvuII, TaqI.
References

Smith M, et al. Mapping of a gene determining tuberous sclerosis to human chromosome 11q14-11q23. Genomics 6: 105-114, 1990. PubMed: 2303253

Charmley P, et al. A primary linkage map of the human chromosome 11q22-23 region. Genomics 6: 316-323, 1990. PubMed: 2307473

Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence MCT128.1 on chromosome 11 [D11S285]. Nucleic Acids Res. 16: 378, 1988. PubMed: 2893342

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