| Comments |
Restriction digests of the clone give the following sizes (kb): BglII--13.0, 9.5, 6.8, 4.4, 2.8, 2.6, 2.5 (doublet), 1.4, 1.3, 1.2, 0.6, 0.3; NotI--40.6, 8.3; BamHI--17.0, 12.0, 9.0, 8.0, 3.7, 1.7; EcoRI--16.0, 14.0, 9.0, 7.8, 2.6, 1.08, 0.8; HindIII--8.6, 6.6, 6.2, 4.0, 3.8, 3.7, 3.6, 3.3, 2.5, 2.4, 2.1, 1.2, 0.9, 0.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: MspI, PstI. |
| References |
Julier C, et al. A detailed genetic map of the long arm of chromosome 11. Genomics 7: 335-345, 1990. PubMed: 1973139
Julier C, et al. Isolation and mapping of a polymorphic DNA sequence (CJ52.15) on chromosome 11 [D11S383]. Nucleic Acids Res. 17: 10149, 1989. PubMed: 2574851
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