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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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pXPGK-RI0.9
pXPGK-RI0.9
規格:
價格:
編號:B198175
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pXPGK-RI0.9
商品貨號
B198175
Designations
pXPGK-RI0.9
Species
Homo sapiens, human
培養基
Depositors
JM Puck
Applications
The smaller EcoRI fragment has been used as the probe.
Vector
Construct size (kb): 5.260000228881836
Insert
DNA: genomic
Insert lengths(kb): 0.8999999761581421
Gene product: phosphoglycerate kinase 1 [PGK1]
Alleles: A1, A1, A2, A2
Insert Size (kb)
0.900
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 0.74; PvuII--4.9; EcoRI/PvuII--2.4, 1.5, 0.79; BamHI--uncut; HindIII--uncut; AccI--3.3, 1.7.
This PstI RFLP is in strong linkage disequilibrium (87% of maximal) with the 5' BglI RFLP detected at this locus by pSPT-PGK.
Insert contains the last 83 bp of exon 4 and extends 820 bp into the fourth intron of human PGK1.
Occasionally a faint 5.2 kb autosomal band is detected with the PstI RFLP.
The smaller EcoRI fragment has been used as the probe.
Based on the restriction data and the tetracycline sensitivity of this construct, the vector must not be the reported pBR322.
References

Smead DL, et al. RFLP's in human X-linked PGK1: A new probe for the PstI RFLP demonstrates strong linkage disequilibrium with the BglI RFLP. Nucleic Acids Res. 17: 7551, 1989. PubMed: 2571982

Merry DE, et al. Choroideremia and deafness with stapes fixation: a contiguous gene deletion syndrome in Xq21. Am. J. Hum. Genet. 45: 530-540, 1989. PubMed: 2491012

Jennifer M Puck, personal communication

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