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pCMV5-hpbr [pCMV-5PBR, pCMV5-PBR]
pCMV5-hpbr [pCMV-5PBR, pCMV5-PBR]
規格:
價格:
編號:B198798
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCMV5-hpbr [pCMV-5PBR, pCMV5-PBR]
商品貨號
B198798
Designations
pCMV5-hpbr [pCMV-5PBR, pCMV5-PBR]
GenBank Number

M36035

培養基
Species
Homo sapiens, human
Depositors
JF Strauss
Applications
in another host, produces protein benzodiazapine receptor (peripheral)
Vector
Construct size (kb): 5.550000190734863
Insert
DNA: cDNA
Insert lengths(kb): 0.8510000109672546
Tissue: liver
Gene product: benzodiazapine receptor (peripheral) [BZRP]
Target Gene: benzodiazapine receptor (peripheral)
Insert Size (kb)
0.851
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.7, 0.85; BglI--1.6, 1.25, 0.95, 0.68, 0.45, 0.35, 0.3; KpnI--5.1, 0.5; PstI--5.0, 0.45, 0.1; SacI--5.0, 0.9.
The insert contains the following restriction sites (bp from the 5' end): BglI--212, 519; KpnI--470; PstI--83, 202, 623; SacI--64; SmaI--761.
BZRP has been localized to 22q13.31 on the human chromosome.
a human genomic EcoRI fragment of approximately 7 kb, and a human genomic HindIII fragment of 6.5 kb. The hamster genomic EcoRI fragment is larger.
Contains the complete coding sequence which is identical to that reported in GenBank accession M36035. This insert also has a longer 5' untranslated region.
Detects RNA of approximately 1 kb in Northern blots,
The insert was cloned into a shuttle vector that regulates expression of inserts by the cytomegalovirus (CMV) major early immediate promoter and contains the human growth hormone (GH) terminator.
References

Chang YJ, et al. The human "pripheral-type" benzodiazepine receptor: regional mapping of the gene and characterization of the receptor expressed from cDNA. DNA Cell Biol. 11: 471-480, 1992. PubMed: 1326278

Riond J, et al. Molecular cloning and chromosomal localization of a human peripheral-type benzodiazepine receptor. Eur. J. Biochem. 195: 305-311, 1991. PubMed: 1847678

Jerome F Strauss, personal communication

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