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pBlueBac/PKC-alpha
pBlueBac/PKC-alpha
規格:
價格:
編號:B198862
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pBlueBac/PKC-alpha
商品貨號
B198862
Designations
pBlueBac/PKC-alpha
GenBank Number

X52479

培養基
Species
Homo sapiens, human
Depositors
DJ Burns
Applications
in another host, produces protein protein kinase C, alpha
Vector
Construct size (kb): 16.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBlueBac
Intact vector size: 14.000
Type of vector: phagemid
Vector end: NheI
Vector end: NheI
Cloning sites: NheI
Polylinker sites:
Host range: insect cells; Escherichia coli
Features (with orientation and position when available):
insert detection: lac
marker(s): ampR
promoter: polyhedrin
replicon: pMB1, f1, SV40
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKCA
Other gene symbols: PKCA
Genomic copy number: unique
Gene name: protein kinase C, alpha
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q22-q24
Tissue: liver, HepG2 cell line
Type of DNA: cDNA
Insert end: SpeI
Insert end: SpeI
Insert size (kb): 2.0
Cross references: DNA Seq. Acc.: X52479
Insert lengths(kb): 2.0
Tissue: liver, HepG2 cell line
Gene product: protein kinase C, alpha [PKCA]
Target Gene: protein kinase C, alpha
Insert Size (kb)
2.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--10.0, 3.5, 1.3, 1.1, 0.25; BglI--5.8, 5.4, 2.2, 1.6, 1.2; NcoI--16.0; PstI--15.0, 0.65; PvuII--3.3, 2.5 (doublet), 2.3, 1.6, 1.4, 1.2, 0.75, 0.35.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.56, 0.77; BglI--0.94; NcoI--1.15; PstI--0.15; PvuII--1.21, 1.93; SmaI--0.02.
Contains the complete coding sequence only (nt 28-2046 of GenBank X52479).
The NheI/SpeI sites were destroyed during cloning.
References

Finkenzeller G, et al. Sequence of human protein kinase C alpha. Nucleic Acids Res. 18: 2183, 1990. PubMed: 2336401

David J Burns, personal communication

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