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當前位置: 首頁 > ATCC代理 > pHRnES1.9 B199402
pHRnES1.9
pHRnES1.9
規格:
價格:
編號:B199402
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pHRnES1.9
商品貨號
B199402
Designations
pHRnES1.9
Species
Homo sapiens, human
培養基
Vector
Construct size (kb): 4.599999904632568
Insert
DNA: genomic
Insert lengths(kb): 1.899999976158142
Gene product: renin [REN]
Alleles: H1, H2, J1, B1, B2, F1, F2, G1, K1, K2, G2, I1
Insert Size (kb)
1.900
Related Products
component of:ATCC 77175
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--2.7, 1.9; HindIII--4.6; PstI--4.6; BamHI--4.6.
Evidence of linkage (theta = 0.02, LOD score = 9.09) was found between the renin (REN) gene on 1q and Van der Woude syndrome (VWS).
This probe is unique in sequence and contains exons 7 - 10 of human renin. There is a short 9 bp exon which has been identified that lies between exon 5 and the former exon 6.
BglI, HindIII, BglII, TaqI, RsaI, and BstXI RFLPs are detected with a mix of the inserts from pHRnX0.8 (ATCC 57174), pHRnX3.6 (ATCC 57178), and pHRnES1.9 (ATCC 57176), apparently from base changes not within the coding regions of the gene.
Detection of the HindIII polymorphism (alleles 8.7 and 6.2 kb) was verified by CEPH.
Enzyme(s) not detecting polymorphism: EcoRI, BanI, MlnI, BstEII, HphI, MspI, SstI, DraI, PvuII, MboII, ApaI, SinI, NciI, ScaI, XbaI, HinfI, NdeI, HincII. XmnI and PstI give uninterpretable results. Tested on a small sample.
References

Naftilan AJ, et al. A lack of genetic linkage of renin gene restriction fragment length polymorphisms with human hypertension. Hypertension 14: 614-618, 1989. PubMed: 2573574

Murray JC, et al. Linkage of an autosomal dominant clefting syndrome (Van der Woude) to loci on chromosome 1q. Am. J. Hum. Genet. 46: 486-491, 1990. PubMed: 2309700

Chirgwin JM, et al. Human renin gene is on chromosome 1. Somatic Cell Mol. Genet. 10: 415-421, 1984. PubMed: 6379904

Buetow KH, et al. A detailed multipoint gene map of chromosome 1q. Genomics 8: 13-21, 1990. PubMed: 1981991

Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294

Hobart PM, et al. Human renin gene: Structure and sequence analysis. Proc. Natl. Acad. Sci. USA 81: 5026-5030, 1984. PubMed: 6089171

John M Chirgwin, personal communication

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