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pE7
pE7
規(guī)格:
貨期:
編號(hào):B199430
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pE7
商品貨號(hào) B199430
Designations pE7
GenBank Number

K01885

Species Homo sapiens, human
Depositors GT Merlino, I Pastan
Applications
Codominant segregation of the BglII RFLP was observed in 7 families (32 individuals).
For the HincII polymorphism, 9 constant bands smaller than 7.6 kb have been observed.
Vector
Construct size (kb): 6.800000190734863
Insert
DNA: cDNA
Insert lengths(kb): 2.400000095367432
Tissue: epidermoid carcinoma A431 cell line
Gene product: epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog) [EGFR]
Alleles: A3, A3, B1, B1, B2, B2, A1, A1, A2, A2
Insert Size (kb) 2.400
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): ClaI--4.4, 2.4; EcoRI--5.2, 1.7; HindIII--7.0.
Codominant segregation of the BglII RFLP was observed in 7 families (32 individuals).
This is an internal cDNA fragment encoding about 709 amino acids. The 3' end is determined by the ClaI site of the cDNA.
For the HincII polymorphism, 9 constant bands smaller than 7.6 kb have been observed.
Enzyme(s) not detecting polymorphism: MspI, TaqI, EcoRI.
References

Motomura K, et al. A new BglII RFLP at the epidermal growth factor receptor locus (erbB-1) on chromosome 7. Nucleic Acids Res. 17: 2369, 1989. PubMed: 2565027

Merlino GT, et al. Structure and localization of genes encoding aberrant and normal epidermal growth factor receptor RNAs from A431 human carcinoma cells. Mol. Cell. Biol. 5: 1722-1734, 1985. PubMed: 2991749

Xu YH, et al. Human epidermal growth factor receptor cDNA is homologous to a variety of RNAs overproduced in A431 carcinoma cells. Nature 309: 806-810, 1984. PubMed: 6330563

Korc M, et al. Enhanced expression of epidermal growth factor receptor correlates with alterations of chromosome 7 in human pancreatic cancer. Proc. Natl. Acad. Sci. USA 83: 5141-5144, 1986. PubMed: 3014534

Abrahamson G, Wainscoat JS. A new HincII polymorphism at the epidermal growth factor receptor locus on chromosome 7. Nucleic Acids Res. 18: 3104, 1990. PubMed: 1971927

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