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pH9RA3
pH9RA3
規格:
價格:
編號:B199803
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pH9RA3
商品貨號
B199803
Designations
pH9RA3
GenBank Number

M84747

培養基
Species
Homo sapiens, human
Depositors
J Renauld
Applications
in another host, produces protein interleukin 9 receptor
Vector
Construct size (kb): 7.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pRc/RSV
Intact vector size: 5.100
Type of vector: phagemid
Vector end: BstXI
Vector end: BstXI
Cloning sites: HindIII SpeI BstXI NotI XbaI
Polylinker sites: HindIII KpnI SacI BamHI SpeI XmaIII BstXI EcoRI PstI EcoRV
BstXI NotI XhoI SphI NsiI XbaI
Construction: pUC19, RSV, bovine growth hormone
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, neoR, G418R
promoter: RSV LTR
replicon: pMB1, M13
terminator: BGH polyadenylation
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IL9R
Gene name: interleukin 9 receptor
Contains complete coding sequence?: Y
Tissue: megakaryoblastic leukemia Mo7E cell line
Type of DNA: cDNA
Insert end: BstXI
Insert end: BstXI
Insert size (kb): 2.3
Cross references: DNA Seq. Acc.: M84747
Insert lengths(kb): 2.299999952316284
Tissue: megakaryoblastic leukemia Mo7E cell line
Gene product: interleukin 9 receptor [IL9R]
Target Gene: interleukin 9 receptor
Insert Size (kb)
2.300
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII/NotI--5.4, 2.5; BamHI--3.0, 2.85, 2.1; PstI--6.4, 1.5; SmaI--5.2, 2.75; EcoRI--4.4, 3.5.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.08, XbaI--0.33; SfiI--1.17; SmaI--1.33; SacI--1.34, 1.57, 2.25.
Contains the complete coding sequence (nt 180-1748) plus 5' and 3' untranslated regions.
The 5' end of the insert is adjacent to the unique HindIII and SpeI sites of the MCS; the 3' end of the insert is adjacent to the unique NotI site of the MCS.
References

Renauld JC, et al. Expression cloning of the murine and human interleukin 9 receptor cDNAs. Proc. Natl. Acad. Sci. USA 89: 5690-5694, 1992. PubMed: 1376929

Jean-Christophe Renauld, personal communication

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