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pUK21deltaBB
pUK21deltaBB
規(guī)格:
貨期:
編號(hào):B201259
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pUK21deltaBB
商品貨號(hào) B201259
Designations pUK21deltaBB
Depositors DJ Stillman
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101
Vector Information
Size (kb): 3.0000000000000000
DESCRIPTION OF VECTOR:
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: StuI XhoI EcoRV NotI XbaI ClaI
Polylinker sites: SpeI StuI XhoI EcoRV NotI XbaI SpeI ClaI
Construction: pUC
Features (with orientation and position when available):
Vector: pUK21deltaBB (plasmid)
Construction: pUC21
Marker(s):kanR
Construct size (kb): 3.0
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRV--3.0; PvuI--1.7, 1.3; XbaI--3.0.
The plasmid derived from pUK21 described by deleting the BamHI-BglII fragment.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
Protocol: (LB + kan)
References

Vieira J. New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100: 189-194, 1991. PubMed: 1905257

Voth WP. Yeast vectors for integration at the HO locus.. Nucleic Acids Res. 29(12): E59-9, 2001. PubMed: 11410682

David J Stillman, personal communication

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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