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Hf32
Hf32
規(guī)格:
價格:詢價
編號:B201378
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Hf32
商品貨號
B201378
Designations
Hf32
Species
Homo sapiens, human
培養(yǎng)基
Vector
Construct size (kb): 6.5
Insert
DNA: cDNA
Insert lengths(kb): 2.099999904632568
Tissue: skin fibroblast
Gene product: collagen, type I, alpha 2 [COL1A2]
Alleles: A1, B1, K1, C1, A2, C2, K2, b2
Insert Size (kb)
2.100
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
The 5' EcoRI site was lost in cloning. The insert contains the following sites (in approximate kb from the 5' end): AvaI--0.3; PvuII--0.5, XhoI--0.75; AvaI--0.8; PstI--1.15; BglI-1.35; XbaI--1.55; SacI--1.85.
Restriction digests of the clone give the following sizes (kb): HindIII--6.5; EcoRI--6.5; PstI--4.6, 1.8; BamHI--6.5; SalI--6.5; HindIII/SstI--4.2, 1.2, 1.1.
This insert codes for 480 residues of the collagenous region. It also encodes most of the COOH-terminal propeptide.
This clone detects major mRNA species of about 5.1 and 4.6 kb in human skin fibroblasts and a minor transcript of about 4.4 kb.
References

Myers JC, et al. Cloning a cDNA for the pro-alpha2 chain of human type I collagen. Proc. Natl. Acad. Sci. USA 78: 3516-3520, 1981. PubMed: 6267597

Bernard MP, et al. Structure of a cDNA for the proalpha2 chain of human type I procollagen. Comparison with chick cDNA for proalpha2(I) identifies structurally conserved features of the protein and the gene. Biochemistry 22: 1139-1145, 1983. PubMed: 6687691

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