Comments
Restriction digests of the clone give the following sizes (kb): BamHI--9.0; EcoRI--6.5, 1.8, 0.58; HindIII--9.0; KpnI--6.5, 2.0, PstI--4.5, 2.1(doublet), 0.31.
Restriction digests of the clone give the following sizes (kb): BamHI--9.0; EcoRI--6.5, 1.8, 0.58; HindIII--9.0; KpnI--6.5, 2.0; PstI--4.5, 2.1 (doublet), 0.31.
Digestion with EcoRI yields two fragments of 2.0 and 0.5 kb.
The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.2, 0.4, 0.7, 0.75; EcoRI--1.9; HindIII--1.2; KpnI--1.0; NcoI--0.3, 1.75; PstI--0.5; PvuI--2.0; PvuII--0.05, 0.6; SacI--0.5, 1.2.
Encodes aa 160-911 of the mutant form: K816I, D819F, E820F and Leu insertion at aa 821.
Cross hybridizes with a 6.8 kb rat liver mRNA.
pKCR-2 is an expression vector containing SV40 early and late promoters and beta-globin splicing and polyadenylation signals.
