ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.7, 0.64; EcoRI--3.0, 1.35; HindIII--4.4; PstI--3.3, 0.5, 0.27; XbaI--4.4.
The insert contains the following restriction sites (nt from the 5' EcoRI site): XmnI--72, AccI--142, BglII--159, PstI--318; BamHI--634; NarI--916; SmaI--1060.
This clone encodes the synthesis of a 50 kDa fusion protein recognized by anti-NADPH oxidase antisera.
Detects a mRNA of 1.4 kb.
References
Volpp BD, et al. Cloning of the cDNA and functional expression of the 47-kilodalton cytosolic component of human neutrophil respiratory burst oxidase. Proc. Natl. Acad. Sci. USA 86: 7195-7199, 1989. PubMed: 2550933
Volpp BD, et al. Two cytosolic neutrophil oxidase components absent in autosomal chronic granulomatous disease. Science 242: 1295-1297, 1988. PubMed: 2848318
. Medical sciences: correction. Proc. Natl. Acad. Sci. USA 86: 9563, 1989.
Casimir CM, et al. Autosomal recessive chronic granulomatous disease caused by deletion at a dinucleotide repeat. Proc. Natl. Acad. Sci. USA 88: 2753-2757, 1991. PubMed: 2011585
