| Comments |
Restriction digests of the clone give the following sizes (kb): SalI--26.0, 8.4, 2.5, 2.4, 0.8; AvaI--8.0, 7.0, 6.2, 3.6, 3.4, 1.6 (doublet), 1.0 + smaller; NotI-->23.0, 9.0, 8.0; BamHI--10.5, 9.0 (doublet), 5.6, 4.4, 2.6, 2.1, 1.25; EcoRI--13.0, 9.0 (doublet), 8.0, 2.9, 1.6, 0.9, 0.7; HindIII--16.0, 15.0, 7.0, 3.6, 1.5. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: MspI, PstI. |
| References |
Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986
Julier C, et al. Isolation and mapping of a polymorphic DNA sequence (CJ52.112) on chromosome 16 [D16S158]. Nucleic Acids Res. 17: 8019, 1989. PubMed: 2477807
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