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PRDII-BF1
PRDII-BF1
規格:
價格:
編號:B204033
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
PRDII-BF1
商品貨號
B204033
Designations
PRDII-BF1
GenBank Number

X51435

培養基
Species
Homo sapiens, human
Depositors
C Fan, T Maniatis
Applications
The encoded protein binds to the HIV-1 enhancer, the MHC H2-Kb promoter, and the immunoglobulin kappa enhancer.
Vector
Construct size (kb): 13.5
Insert
DNA: cDNA
Insert lengths(kb): 9.0
Gene product: human immunodeficiency virus type I enhancer-binding protein 1(zinc finger protein 40 (major histocompatibility complex binding protein 1, positive regulatory dom) [ZNF40]
Alleles: A1, A1, A1, A2, A2, A2
Insert Size (kb)
9.0
Media
ATCC® Medium 1235: LB Medium (ATCC medium 1065) with 5 mcg/ml tetracycline and 10 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.1, 3.9, 2.8, 1.6; EcoRI--6.5, 2.7 (doublet), 1.6; HindIII--10.9, 1.8, 0.7; PstI--4.4, 2.2 (doublet), 1.3, 1.2 (doublet), 1.0 + smaller; XbaI--6.8, 4.3, 2.1.
The insert contains the following restriction sites (nt position according to published sequence): BamHI--2067, 3633, 7529; EcoRI--2184, 3828, 6430; HindIII--1917, 2569, 2588.
The encoded protein binds to the HIV-1 enhancer, the MHC H2-Kb promoter, and the immunoglobulin kappa enhancer.
Detects genomic HindIII fragments of the following sizes (approximate kb): human--17, 4.9, 3.0; mouse--10.
Extends from the NotI site at nt 152 (blunted) to a SmaI site (blunted) beyond nt 9015. Contains the complete coding sequence and a poly(A) tail of approximately 100 nt.
The MspI polymorphism was detected using the 1.6 kb EcoRI fragment.
References

Gaynor RB, et al. Localization of the zinc finger DNA-binding protein HIV-EP1/MBP-1/PRDII-BF1 to human chromosome 6p22.3-p24. Genomics 9: 758-761, 1991. PubMed: 2037300

Fan CM, Maniatis T. A DNA-binding protein containing two widely separated zinc finger motifs that recognize the same DNA sequence. Genes Dev. 4: 29-42, 1990. PubMed: 2106471

Fountain JW, et al. MspI RFLP detected by a ZNF-40 cDNA sequence. Nucleic Acids Res. 19: 2514, 1991. PubMed: 1710360

Chen-Ming Fan, personal communication

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