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lambdaS12 [lambdaSgamma12, pcD-GALT]
lambdaS12 [lambdaSgamma12, pcD-GALT]
規格:
價格:
編號:B204206
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
lambdaS12 [lambdaSgamma12, pcD-GALT]
商品貨號
B204206
Designations
lambdaS12 [lambdaSgamma12, pcD-GALT]
GenBank Number

M13429

培養基
Species
Homo sapiens, human
Depositors
M Lefranc, TH Rabbitts
Applications
A 1.2 kb SacI/EcoRI fragment containing the Vgamma3-Jgamma2 rearranged gene (S12SR, ATCC 59590, 59591) has been used to detect rearrangements.
Vector
Construct size (kb): 45.5
Insert
DNA: genomic
Insert lengths(kb): 16.0
Tissue: T-lymphoma SUP-T1 cell line
Gene product: T cell receptor, gamma, variable region V3 [TCRGC2]
Alleles: B1, D4, J2, D2, A1, A2, B2, G1, G2, J1, D1, D3
Insert Size (kb)
16.0
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--20.0, 15, 3.7, 3.6, 0.5; KpnI--19.0, 17.0, 3.9, 2.9, 1.9, 1.6; PvuI--23.0, 12.5;
SacI--20.0, 9.4, 8.0, 4.4, 2.9, 1.5, 0.8; SacI/EcoRI--20.0, 9.4, 6.6, 4.0, 1.3, 0.9, 0.6; XhoI--20.0, 18.0, 9.0.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--4.6, 12.0, 14.8, 16 (polylinker site); HindIII--3.0, 3.5, 7.2, 10.8.
A 1.2 kb SacI/EcoRI fragment containing the Vgamma3-Jgamma2 rearranged gene (S12SR, ATCC 59590, 59591) has been used to detect rearrangements.
Contains a productive rearrangement of Vgamma3 and Jgamma2. The insert begins about 0.5 kb upstream of Vgamma2 and extends 2.5 kb beyond exon 2R of TCRGC2.
The insert extends 1.2 kb more in the 5' direction and is 1.8 kb shorter at the 3' end than lambdaS1.
Additional GenBank/EMBL accession numbers: X03437, M13824.
References

Lefranc MP, et al. Rearrangement of two distinct T-cell gamma-chain variable-region genes in human DNA. Nature 319: 420-422, 1986. PubMed: 3003579

LeFranc MP, et al. Diversity and rearrangement of the human T cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45: 237-246, 1986. PubMed: 2938743

Marie-Paule Lefranc, personal communication

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