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TeloHAEC-GFP
TeloHAEC-GFP
規(guī)格:
價(jià)格:
編號(hào):B204361
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
TeloHAEC-GFP
商品貨號(hào)
B204361
Organism
Homo sapiens, human
Tissue
aorta
培養(yǎng)基
Cell Type
endothelial
Product Format
frozen
Morphology
endothelial
Culture Properties
adherent
Biosafety Level
2  [Cells containing SV40 viral DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
normal
Age
34 years old
Gender
female
Applications

TeloHAEC-GFP (ATCC CRL-4054) cells retain important endothelial cell characteristics such as  CD31/PECAM-1 marker expression and LDL functional uptake; the cells also show effective inflammatory response upon TNFα treatment and increase proliferation upon VEGF stimulation.  When co-cultured with fibroblasts, TeloHAEC cells can also form neoangiogenic tubular networks in vitro, which are responsive to VEGF stimulation and suramin inhibition.

Storage Conditions
liquid nitrogen vapor phase
Karyotype
This is a diploid cell line of female origin with a consistent normal kayrotype at low and high passages
Images
CRL-4054 Cell Micrograph CRL-4054 TeloHaec-GFP CD31 Positive CRL-4054 TeloHaec-GFP AcLDL Uptake CRL-4054 TeloHaec-GFP co-cultured with hTERT MSC - Tubulogenesis
Derivation
TeloHAEC-GFP is a clonal cell line by stably expressing EmGFP under EF1α promoter in TeloHAEC (ATCC CRL-4052) cell line.
Clinical Data
female 

34

Antigen Expression
Positive for CD31/PECAM-1 expression  and capable of uptaking Low Density Lipoprotein (LDL).
Complete Growth Medium
The base medium for this cell line is Vascular Cell Basal Medium (ATCC PCS-100-030), supplemented with Vascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041).   Optional: Add 0.3ug/mL Puromycin (Santa Cruz Biotech: sc-108071A). Note: Do not filter complete medium.
Subculturing
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes.

Subculture when the culture is about 90% confluent.
  1. Remove and discard spent medium.
  2. Briefly rinse the cells with Dulbecco's Phosphate Buffered Saline (DPBS, ATCC 30-2200) and discard rinse solution.
  3. Add 2.0 to 3.0 mL room temperature Trypsin-EDTA for Primary Cells (ATCC PCS-999-003) to the flask. Incubate at 37°C for 5 min (until cells have detached).
  4. Neutralize trypsin by adding an equal volume of room temperature 2% FBS in DPBS.
  5. Centrifuge cells at 250 x g for 5 min at room temperature.
  6. Remove supernatant. Resuspend pellet in 6.0 to 8.0 mL Complete Growth Medium.
  7. Count cells, and seed 5.0 x 103 to 8.0 x 103 viable cells/cm2 to new culture vessels.
Medium Renewal: Every 2-3 days.
Cryopreservation
90% FBS, 10% DMSO
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile

D5S818:  12        
D13S317: 9, 12
D7S820: 10, 11        
D16S539: 12, 13
vWA: 15, 16             
Amelogenin:  X
TPOX: 8
CSF1PO: 11, 12
TH01: 6, 8
Population Doubling Level (PDL)
As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation.
Name of Depositor
ATCC
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