Vector provides selection markers for transformation of Saccharomyces cerevisiae or other eukaryotes based on G418 resistance.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Rao TR, Reddy CA. A YIp5-kanR plasmid useful for isolating ars from yeast and other eukaryotes based on G418 resistance selection. Nucleic Acids Res. 14: 7504, 1986. PubMed: 3532035
Struhl K, et al. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc. Natl. Acad. Sci. USA 76: 1035-1039, 1979. PubMed: 375221