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pKi-67/1
pKi-67/1
規格:
價格:
編號:B204523
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pKi-67/1
商品貨號
B204523
Designations
pKi-67/1
GenBank Number

X65550

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 6.800000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR325
Intact vector size: 5.996
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI
EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmLR, tetR
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MKI67
Gene name: antigen identified by monoclonal antibody Ki-67
Contains complete coding sequence?: N
Chromosome: 10; Localization: 10 q25-qter
Tissue: IM-9 cell line
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.092
Cross references: DNA Seq. Acc.: X65550
Insert lengths(kb): 1.092000007629395
Tissue: IM-9 cell line
Gene product: antigen identified by monoclonal antibody Ki-67( Proliferation-related Ki-67 antigen, Ki-67 antigen) [MKI67]
Insert Size (kb)
1.092
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.0, 1.1; HindIII--7.0; PstI--5.0, 2.1; SacI--7.1; XbaI--7.1.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--1.00; SacI--0.21, 0.57, 0.73, 0.94; XbaI--0.18.
It includes three of sixteen Ki-67 repeats.
Detects splicing isoforms of 8.7 and 9.8 kb.
References

Fonatsch C, et al. Assignment of the human Ki-67 gene (MKI67) to 10q25-qter. Genomics 11: 476-477, 1991. PubMed: 1769665

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