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pCMV5.15
pCMV5.15
規(guī)格:
價格:
編號:B204942
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pCMV5.15
商品貨號
B204942
Designations
pCMV5.15
GenBank Number

J00136

培養(yǎng)基
Species
Homo sapiens, human
Applications
in another host, produces protein coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B)
Vector
Construct size (kb): 7.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCMV5
Intact vector size: 4.700
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI BamHI HindIII
Polylinker sites:
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
promoter: CMV, ->
terminator: hGH, ->
replicon: SV40
replicon: pMB1
marker(s): ampR, <-
replicon: f1, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: F9
Genomic copy number: unique
Gene name: coagulation factor IX (plasma thromboplastic component,
Christmas disease, hemophilia B)
Contains complete coding sequence?: U
Chromosome: X; Localization: X q26.3-q27.1
Type of DNA: cDNA
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 2.8
Cross references: DNA Seq. Acc.: J00136
Insert lengths(kb): 2.799999952316284
Gene product: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) [F9]
Alleles: A1, A2, D2, C1, D1, C2
Target Gene: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B)
Insert Size (kb)
2.800
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 3.0; SstI--7.2, 0.5; NotI--7.4; BstEII--7.4; EcoRI--5.0, 2.6.
Digestion with EcoRI produces fragments of 5.0 and 2.5 kb.
Bacteria may grow slowly due to the high plasmid copy number.
Encodes a chimera in which the second epidermal growth factor-like domain was replaced with the corresponding sequence from factor X.
References

Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778

Cheung WF, et al. The role of the epidermal growth factor-1 and hydrophobic stack domains of human factor IX in binding to endothelial cells. J. Biol. Chem. 266: 8797-8800, 1991. PubMed: 1851159

Lin SW, et al. Expression and characterization of human factor IX and factor IX - factor X chimeras in mouse C127 cells. J. Biol. Chem. 265: 144-150, 1990. PubMed: 2294100

Wing-Fai Cheung, personal communication

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