| 產品名稱 |
pBS-NC4.5 |
| 商品貨號 |
B205229 |
| Designations |
pBS-NC4.5 |
| Species |
Tomato spotted wilt virus |
| Depositors |
TL German |
| Applications |
contains sequence |
| Vector |
Construct size (kb): 4.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: PstI
Vector end: PstI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: tomato spotted wilt tospovirus
Gene symbol: TSWV N
Contains complete coding sequence?:
Strain: Hawaiian L isolate
Disease: tomato spotted wilt virus
Type of DNA: cDNA
Insert 5' end: PstI
Insert 3' end: PstI
Insert size (kb): 1.5
Cross references: Insert lengths(kb): 1.5 Tissue: tomato spotted wilt virus Disease: tomato spotted wilt virus Gene product: [TSWV N] |
| Insert Size (kb) |
1.5 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--3.0, 1.5; PstI/PvuII--2.5, 0.8, 0.7, 0.3, 0.2; EcoRI--3.3, 1.2; BamHI--4.5. The 1.5 kb insert contains the cauliflower mosaic virus 35S RNA promoter and the nopaline synthase terminator. A PstI/PvuII digest yields an approximately 800 bp band containing the N gene ORF. |
| References |
Kim JW. Disease Resistance in Tobacco and Tomato Plants Transformed with the Tomato Spotted Wilt Virus Nucleocapsid Gene. Plant Dis. 78: 615-621, 1994.
|
