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pBS-NC4.5
pBS-NC4.5
規(guī)格:
價(jià)格:
編號(hào):B205229
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pBS-NC4.5
商品貨號(hào)
B205229
Designations
pBS-NC4.5
Species
Tomato spotted wilt virus
培養(yǎng)基
Depositors
TL German
Applications
contains sequence
Vector
Construct size (kb): 4.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: PstI
Vector end: PstI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: tomato spotted wilt tospovirus
Gene symbol: TSWV N
Contains complete coding sequence?:
Strain: Hawaiian L isolate
Disease: tomato spotted wilt virus
Type of DNA: cDNA
Insert 5' end: PstI
Insert 3' end: PstI
Insert size (kb): 1.5
Cross references:
Insert lengths(kb): 1.5
Tissue: tomato spotted wilt virus
Disease: tomato spotted wilt virus
Gene product: [TSWV N]
Insert Size (kb)
1.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.0, 1.5; PstI/PvuII--2.5, 0.8, 0.7, 0.3, 0.2; EcoRI--3.3, 1.2; BamHI--4.5.
The 1.5 kb insert contains the cauliflower mosaic virus 35S RNA promoter and the nopaline synthase terminator. A PstI/PvuII digest yields an approximately 800 bp band containing the N gene ORF.
References

Kim JW. Disease Resistance in Tobacco and Tomato Plants Transformed with the Tomato Spotted Wilt Virus Nucleocapsid Gene. Plant Dis. 78: 615-621, 1994.

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