Restriction digests of the clone give the following sizes (kb): BamHI--6.3; ClaI/BamHI--3.3, 2.6, 0.5; HindIII/EcoRI--3.3, 2.0, 1.2.
Shuttle expression vector.
Permits expression in both S. cerevisiae and mouse cells.
The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter.
Constructed by replacing the HindIII/EcoRI fragment of pCYM3 with the HindIII/EcoRI multiple cloning site from M13mp11. The translation start codon has been removed by this process.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Camonis JH, et al. Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells. Gene 86: 263-268, 1990. PubMed: 2108907
