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pCYM3-11
pCYM3-11
規(guī)格:
貨期:
編號:B205241
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCYM3-11
商品貨號 B205241
Designations pCYM3-11
Depositors JH Camonis
Biosafety Level 1
Vector Information
Size (kb): 6.4850001335144040
Vector: pCYM3-11 (plasmid)
Promoters: Promoter SV40 early
Construction: pCYM3 (ATCC 37826), M13mp11
Marker(s):TRP1,ampR,URA3d
Construct size (kb): 6.485000133514404
Features: marker(s): ampR, TRP1, URA3d
promoter: SV40 early
replicon: pMB1, 2 micron
terminator: SV40
Applications
YE-type (episomal) shuttle vector
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.3; ClaI/BamHI--3.3, 2.6, 0.5; HindIII/EcoRI--3.3, 2.0, 1.2.
Shuttle expression vector.
Permits expression in both S. cerevisiae and mouse cells.
The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter.
Constructed by replacing the HindIII/EcoRI fragment of pCYM3 with the HindIII/EcoRI multiple cloning site from M13mp11. The translation start codon has been removed by this process.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Camonis JH, et al. Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells. Gene 86: 263-268, 1990. PubMed: 2108907

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