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pNB404

規格:

價格:

編號:B205252

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pNB404

商品貨號

B205252

Designations

pNB404

Depositors

DJ Stillman

培養基

Biosafety Level

Vector Information

Size (kb): 10.0600004196167000

Vector: pNB404 (plasmid)

Promoters: Promoter truncated CYC1 promoter (no UAS element)

Construction: pBR322

Marker(s):URA3,ampR

Construct size (kb): 10.0600004196167

Features: marker(s): URA3
marker(s): ampR
promoter: truncated CYC1 promoter (no UAS element)
replicon: 2 micron ori
replicon: pMB1
coding sequence: lacZ

Applications

YE-type (episomal) shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 3.3, 2.3; XbaI--7.6, 3.8; SalI--11.0.

This is one of three lacZ reporter plasmids that lack UAS promoter elements (ATCC 87509, 87510 & 87513). They differ by polylinker sites.

DNA fragments can be inserted into these plasmids to assay UAS (upstream activation site) activity.

The vector was constructed by Susan Henry.

Growth Conditions

Temperature: 37.0°C

References

Bachhawat N, et al. Functional characterization of an inositol-sensitive upstream activation sequence in yeast. A cis-regulatory element responsible for inositol-choline mediated regulation of phospholipid biosynthesis. J. Biol. Chem. 270: 25087-25095, 1995. PubMed: 7559640

Related Products

component of:ATCC 87539

Shipped

freeze-dried

Shipping Information

?Freeze dried E. coli containing the plasmid

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