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HFBCT78
HFBCT78
規(guī)格:
價(jià)格:
編號(hào):B205469
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
HFBCT78
商品貨號(hào)
B205469
Designations
HFBCT78
GenBank Number

T05242

培養(yǎng)基
Species
Homo sapiens, human
Depositors
JC Venter
Vector
Construct size (kb): 3.799999952316284
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PLA2G2A
Other gene symbols: PLA2L; PLA2B
Gene name: phospholipase A2, group IIA (platelets, synovial fluid)
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.8
Cross references: DNA Seq. Acc.: M86400; EST: EST03131
Nucleotides 1-193 of the insert correspond to
nucleotides 2581-2388 of M86400 with 100% identity.
DNA Seq. Acc.: T05242; EST: EST03131; Primer: M13-21
Insert lengths(kb): 0.800000011920929
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: phospholipase A2, group IIA (platelets, synovial fluid)(phospholipase A2 polypeptide B (synovial fluid)) [PLA2B]
Insert Size (kb)
0.800
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8.
Based on the insert size and the match to the published sequence, probably a partial clone consisting primarily of 3' untranslated region, because the coding sequence of M86400 is nt 85-822.
References

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

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