Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
培養(yǎng)基
Isolation
sediment near shore, Assawoman Bay, Ocean City, MD, 1989
Product Format
frozen
Type Strain
no
Medium
ATCC® Medium 1525: Seawater 802 medium
Growth Conditions
Temperature: 25.0°C
Protocol: ATCCNO: 50419 SPEC: Upon receipt place frozen ampule directly into a 35C water bath. Transfer thawed contents to 10 ml of bacterized ATCC medium 1525 in a T-25 tissue culture flask. The medium is bacterized by adding a loopful of Klebsiella pneumoniae subsp. pneumoniae from an agar slant to 10 ml of medium at least 24 hours before inoculation with the thawed suspension. Subculture every 14 days. Vigorously agitate and aseptically transfer a 0.1 ml aliquot of cell suspension to a fresh flask of bacterized medium.
Subcultivation
Protocol: ATCCNO: 50419 SPEC: Upon receipt place frozen ampule directly into a 35C water bath. Transfer thawed contents to 10 ml of bacterized ATCC medium 1525 in a T-25 tissue culture flask. The medium is bacterized by adding a loopful of Klebsiella pneumoniae subsp. pneumoniae from an agar slant to 10 ml of medium at least 24 hours before inoculation with the thawed suspension. Subculture every 14 days. Vigorously agitate and aseptically transfer a 0.1 ml aliquot of cell suspension to a fresh flask of bacterized medium.
