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p214LT
p214LT
規(guī)格:
價格:
編號:B205605
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
p214LT
商品貨號
B205605
Designations
p214LT
GenBank Number

J02288

培養(yǎng)基
Species
polyomavirus
Depositors
R Kamen
Vector
Construct size (kb): 5.5
Insert
DNA: genomic
Insert lengths(kb): 2.200000047683716
Gene product: large T antigen
Insert Size (kb)
2.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Freeze dried Escherichia coli containing the plasmid.

Comments
Constructed from pPyLT-1 by removing the EcoRI fragment. Stop codon lies in plasmid.
Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BamHI/BglI--2.2, 1.8, 0.8, 0.6, 0.3; BamHI/EcoRI--3.8, 2.2.
The insert contains a truncated portion of the Large T Antigen.
References

Rassoulzadegan M, et al. The roles of individual polyoma virus early proteins in oncogenic transformation. Nature 300: 713-718, 1982. PubMed: 6294529

Robert Kamen, personal communication

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