The insert includes the following restriction sites (nt positions according to the published sequence): EcoRI--1786; PstI--2125; AccI--3130, 3639. Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.8, 0.6; BamHI--5.6; HindIII--5.6; PstI--4.4, 0.9; AccI--2.9, 1.9, 0.5. The insert extends from approximately nt 1250 to approximately nt 3750. The first 16 nt of the insert are of unknown origin. The open reading frame extends from nt 207 to nt 7247. There is approximately 1 kb of 3' untranslated sequence which has not been fully characterized. Subcloned from lambdagt10SII.1. |
