Restriction digests of the clone give the following sizes (kb): BamHI--5.3; XbaI--5.3; PstI--5.3; HindIII--5.3.
This is a Escherichia coli/yeast episomal shuttle vector with multiple restriction sites for cloning.
Constructed by filling in and blunt-end ligating pUC18 linearized at NdeI, a 1.4 kb RsaI fragment of the 2 micron circle, and a 1.1 kb HindIII/SmaI fragment containing URA3. A XbaI site in 2u and a PstI site in URA3 were removed.
Hill JE, et al. Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 2: 163-167, 1986. PubMed: 3333305
Alexander Tzagoloff, personal communication
