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Human rhinovirus 7
Human rhinovirus 7
規(guī)格:
價格:
編號:B205822
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Human rhinovirus 7
商品貨號
B205822
Classification
Picornaviridae, Rhinovirus, Human rhinovirus A
Agent
Human rhinovirus 7
培養(yǎng)基
Strain
68-CV11
Applications
Respiratory research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen
Storage Conditions
?-70°C or colder
Comments
Optimal conditions for grwoth include pH 8.6-7.3 in roller or rocker cultures incubated at approximately 33°C. All members of the rhinovirus group are ether resistant.
Effect on Host
?CPE in culture include cell rounding, clumping, and sloughing.
Recommended Host
Production host - HeLa cells (ATCC CCL-2)
Alternate host(s) - HD, HEK, MRC-5, MkK, WI-38

Recommended Host: ??HeLa cells (ATCC CCL-2?)

Growth Conditions
For best results, plant cells 24-48 hours prior to infection. Infect at 70-80% confluence using a multiplicity of infection (MOI) of 0.1-1. Incubate 1-7 days at 33°C, rocking, in a humidified 5% CO2 atmosphere. Harvest culture fluids or culture fluids plus cells. Freeze as rapidly as possible.
Name of Depositor
NIAID, ATCC
Source
Derived from NIAID reagent V-127-001-021 (VR-117) by passage at ATCC
References

Hamparian VV, et al. Recovery of new viruses (coryzavirus) from cases of common cold in human adults. Proc. Soc. Exp. Biol. Med. 108: 444-453, 1961. PubMed: 13904225

NIAID Catalog of Research Reagents 1978-1980. S. Cunningham, ed. US Department of Health Education and Welfare, Public Health Service, National Institutes of Health; DHEW Pulication No. (NIH) 78-899, Bethesda, MD.

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