Name of construct: pSP65:ClVd#5
Excise insert: EcoRI+HindIII
Size of construct (kb): 3.2
Markers: ampR
Name of vector: pSP65
Intact vector size: 3.010
Type of vector: plasmid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range:Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: SP6
replicon: pMB1
terminator: none
Insert
Genome: Coleus viroid (ClVd)
Gene name: genome, partial
Contains complete coding sequence?: U
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.2
Insert Size (kb)
0.2
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.1, 0.24; BglI--3.3; HindIII--3.3; PvuII--3.3.
