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p78/2-8b
p78/2-8b
規格:
價格:
編號:B205929
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
p78/2-8b
商品貨號
B205929
Designations
p78/2-8b
Species
Homo sapiens, human
培養基
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 2.650000095367432
Tissue: embryonic lung HEL cell line, interferon-treated
Gene product: myxovirus (influenza) resistance 1, homolog of murine (interferon-inducible protein p78)(myxovirus (influenza) resistance 1, homolog of murine (interferon-inducible protein (p78)Y)) [MX]
Alleles: A1, A2, B1, B2, A1, A2, B1, B2
Insert Size (kb)
2.650
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 2.65; MluI--uncut; PstI--3.4, 1.1, 0.9; BamHI--5.6; HindIII--3.1, 2.5.
This probe detects the following genomic restriction fragments (kb): NotI--775, BssHII--600; MluI--1225, 1125; NruI--700, SacII--400; SfiI--380.
This clone extends from the EcoRI site at position 137 (5' untranslated region) to the 3' end at nucleotide 2788, and thus encodes a complete polypeptide.
This clone lacks an alternative exon in the 5' noncoding region (249 to 324) and thus contains a HindIII site at 248. 5 of 10 cDNAs sequenced contain the alternative exon.
The polymorphism has been studied using a 1.23 kb PstI fragment from the upstream non-coding and N-terminal coding sequences.
In 40 CEPH families, the haplotype frequencies are as follows: A1B1--0.45; A1B2--0.1; A2B1--0.32; A2B2--0.13.
There is no significant linkage disequilibrium between the two PstI polymorphisms.
References

Petersen MB, et al. Two PstI DNA polymorphisms adjacent to the human gene for the interferon-induced p78 protein (MX1 gene). Nucleic Acids Res. 17: 7546, 1989. PubMed: 2798118

Horisberger MA, et al. Cloning and sequence analyses of cDNAs for interferon- and virus-induced human Mx proteins reveal that they contain putative guanine nucleotide binding sites: functional study of the corresponding gene promoter. J. Virol. 64: 1171-1181, 1990. PubMed: 2154602

Gardiner K, et al. Analysis of human chromosome 21: correlation of physical and cytogenetic maps; gene and CpG island distributions. EMBO J. 9: 25-34, 1990. PubMed: 2295313

Goetschy JF, et al. Regulation of the interferon-inducible IFI-78K gene, the human equivalent of the murine Mx gene, by interferons, double-stranded RNA, certain cytokines, and viruses. J. Virol. 63: 2616-2622, 1989. PubMed: 2542574

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